T cell activation in skin explants from patients with psoriasis and controls

We have worked on skin explants and activated T cells locally with a CD3 antibody, whole biopsies were activated, then epidermal and dermal RNA was sequenced. Sequencing was performed by BGI (Hong Kong) as well as the group analysis. Examination of skin tissue responses to local T cell activation (acd3) compared to isotype at the RNA level. Samples include biopsies from healthy controls and patients with psoriasis treated with Ustekinumab or untreated lesional psoriasis. All biopsy samples were treated either with acd3 or with isotype as indicated.