Capra sibirica Genome sequencing and assembly

The ancient mitochondrial genome of Siberian ibex (Capra sibirica) coded as TCD06G from Tangchaodun Ruins was obtained by high throughput sequencing. The damage pattern demonstrated the authenticity and reliability of the ancient DNA data. The length of the mitogenome was 16,583 bp, which contained 13 protein-coding genes, two rRNA genes, 22 tRNA genes, one replication origin for the L-strand, and one control region. The total base composition of the mitochondrial genome is 31.94% A, 25.62% T, 12.48% G, 25.08% C, and 4.88% missing data with an AT composition of 57.56%. A maximum-likelihood phylogenetic tree was recovered including other genus Capra sequences under the HKY+I+G4 model. This study presents molecular evidence indicates that at least 650 years ago, the northern lineage of Capra sibirica had already been distributed in this region and has persisted to the present day. Additionally, its close genetic relationship with modern Capra sibirica suggests they share similar genetic backgrounds and stable genetic architectures.