FOXC2 knockdown in MDA-MB-231 cells. FOXC2 knockdown in MDA-MB-231 cells

To determine gene expression changes in vasculogenic mimicry competent human breast cancer cells with loss of FOXC2 we performed RNA-seq of MDA-MB-231 cells with FOXC2 knockdown. Overall design: MDA-MB-231 cells were transduced with a control shRNA (shREN) or two different FOXC2-targetting shRNAs (shFOXC2#1 and shFOXC2#3). Two biological replicates per shRNA were subjected to RNA-seq analysis.