Transwell data

Migration
and invasion assays were performed using Transwell chambers (3422, 24-well
insert; 8-μm pore size; Corning Costar, NY, USA) coated with or without
Matrigel (356234, BD Biosciences, NJ, USA). 4 × 105 HNSCC cells
in DMED medium were placed into the upper chamber, and medium supplementary
with 10 % FBS was added to the lower chamber. Then PCI-37A cells were incubated
for 24 hours for migration and 48 hours for invasion, and PCI-37A cells were
incubated for 8 hours for migration and 24 hours for invasion. Then the migratory
or invasive cells were fixed by 4% paraformaldehyde, stained with 0.1% crystal
violet, and counted in three random fields under 200x magnification. Transwell migration assay and Martrigel invasion assay
showed that an ectopic increased level of miR-92b could raise the migration and
invasion ability of PCI-37A cells, while a reduced level of miR-92b led to a
significant decrease in the number of migrating or invasive cells.After
knocked down with SP1 expression, PCI-37A/B cells showed a significant decrease
in migration and invasion.