IQTREEBayesTREE

Sistotrema muscicola was used as the outgroup. The workflow included: (1) Independent alignments of the ITS, nrLSU, and tef1-αsequences were aligned in batches with MAFFT v7.505 (Katoh and Standley, 2013) using auto strategy and normal alignment mode; (2) The sequence alignments were manually adjusted in BioEdit v7.1.3 (Hall 1999). Subsequently, the ITS, nrLSU, and tef1-αregions were concatenated using the concatenate sequence tool in the Alignment toolbar of PhyloSuite v1.2.3 (Xiang et al. 2023); (3) ModelFinder v2.2.0 (Kalyaanamoorthy et al., 2017) was used to select the best-fit model using BIC criterion; (4) Bayesian inference was performed using MrBayes v3.2.7a (Ronquist et al., 2012) with 5 million generations (sampling every 2000 generations, 25% burn-in, and split frequency = 0.004); (5) Maximum likelihood analysis was performed using IQ-TREE v2.2.0 (Nguyen et al. 2015) with 4950 ultrafast bootstraps, as well as the Shimodaira-Hasegawa-like approximate likelihood-ratio test (Guindon et al., 2010); and (6) the tree was visualized for topological structure using the online platform ITOL v7.2.1<br>