Using small RNA-Seq to verify that 5' tRNA-fMet1 half is over-expressed in outer membrane vesicles (OMVs)

5' tRNA-fMet1 half (5'- CGCGGGGTGGAGCAGTCTGGTAGCTCGTCGGGCTC-3') was cloned into the arabinose-inducible expression vector pMQ70. PA14 was transformed with the tRNA-fMet1 half expression vector or empty vector via electroporation. Characterization of the sRNA content of OMVs secreted by 5' tRNA-fMet1 half over-expression clone and empty vector control clone. Overall design: over-expression OMVs (tRNA1-OMVs) and empty vector ctrl OMVs (V-OMVs) from 3 separate preparations.