miR-141-3p regulates the expression of corneal epithelial differentiation

Corneal epithelial RCE1(5T5) cells follow a sequential process that leads to the formation of a 4-5 layered stratified epithelium with a gene expression pattern similar to that shown in primary cultures of corneal epithelial cells. We have previously identified three different developmental stages during the differentiation of the rabbit corneal epithelial cell line RCE1(5T5). In this analysis we describe the participation of miR-141-3p as a regulator of the proliferative phenotype and its participation on maintaining differentiation of corneal eptihelial cells. To evaluate the participation of miR-141 on corneal epithelial differentiation, we obtained the transcriptome of four samples of rabbit corneal epithelium formed by RCE1(5T5) cells at three distinct stages of the differentiation program and RCE1(5T5) cells transfected with antagomiR-141.