Comparative transcriptomics analysis of L-alanine-induced Bacillus subtilis S-2 and 312 strains. Comparative transcriptomics analysis of L-alanine-induced Bacillus subtilis S-2 and 312 strains

Two wide type strains of Bacillus subtilis, S-2 and 312, were selected to study their genic differences treated by L-alanine through comparative transcriptomics analysis. The spores of B. subtilis S-2 were selected because of their high germination potential to L-alanine. The spores of B. subtilis 312 without a response to L-alanine were used as the control. The spores with or without L-alanine (100 mm) pretreatment were both cultured in the synthetic medium for 9 h, and then collected for sequencing. Overall design: Bacillus subtilis S-2, 312, L-alanine-induced S-2 and L-alanine-induced 312 spores were generated by deep sequencing, in duplicate respectively