Immunological properties of neural crest cells derived from human induced pluripotent stem cells (resting state)

Collecting primary neural crest cells (NCCs) of a sufficient quantity for some experiments is difficult, because NCCs are embryonic transient tissue and basically they do not proliferate. We successfully induced NCCs from human induced pluripotent stem cells (iPSCs) according to the previously described method with some modifications. iPSCs-derived NCCs (iPSC-NCCs) are reported to have potential for wound healing and expected to be used clinically in the future. However, nobody reported the immunological properties of iPSC-NCCs. It is important to evaluate the immunological properties of iPSC-NCCs before clinical use. We examined gene expression patterns between iPSCs and iPSC-NCCs. We induced NCCs from human iPSCs according to the previously described method with some modifications for 3 weeks to complete.We extracted total RNA of iPSCs (n=2) and iPSC-NCCs (n=2).