TABLE 1 in New Species of Osedax (Siboglinidae: Annelida) from New Zealand and the Gulf of Mexico

TABLE 1. Genes, primers, references, and PCR reaction protocols used in this study.
GenePrimer setSourceReaction protocolCOIpolyLCO/polyHCO(Carr et al. 2011)95°C/180s–(95°C/40s–42°C/45s–72°C/50s) * 40 cycles–72°C/300sCOIf/COIr(Nelson & Fisher 2000)95°C/300s–(94°C/60s–55°C/60s–72°C/120s) * 35 cycles–72°C/420sLCO1490/HCO2198(Folmer et al. 1994)94°C/180s–(94°C/30s–47°C/45s–72°C/60s) * 5 cycles–(94°C/30s–52°C/45s–72°C/60s) * 30 cycles–72°C/300s16S16SarL/16SbrH(Palumbi 1996)95°C/180s–(95°C/40s–50°C/40s–72°C/50s) * 35 cycles–72°C/300s18S18S-1F/18S-5R(Giribet et al. 1996)95°C/180s–(95°C/30s–50°C/30s–72°C/90s) * 40 cycles–72°C/480s18S-a2.0/18S-9R(Giribet et al. 1996; Whiting et al. 1997)95°C/180s–(95°C/30s–50°C/30s–72°C/90s) * 40 cycles–72°C/480s18S-3F/18S-bi(Giribet et al. 1996; Whiting et al. 1997)95°C/180s–(95°C/30s–52°C/30s–72°C/90s) * 40 cycles–72°C/480s28SD1F/D3R(Brown et al. 1999)94°C/180s–(94°C/60s–55°C/30s–72°C/110s) * 35 cycles–72°C/240sH3H3F/H3R(Colgan et al. 1998)95°C/180s–(95°C/30s–53°C/45s–72°C/45s) * 40 cycles–72°C/300s