Fluctuation of cellular differentiation in limb regeneration is regulated by Pde4b in urodele amphibians

Urodele amphibians, Pleurodeles waltl and Ambystoma mexicanum, have organ-level regeneration capability, such as limb regeneration. Multipotent cells are induced by an endogenous mechanism in amphibian limb regeneration. It is well known that dermal fibroblasts receive regenerative signals and turn into multipotent cells, called blastema cells. However, the induction mechanism of the blastema cells from matured dermal cells was unknown. We previously found that BMP2, FGF2, and FGF8 (B2FF) could play sufficient roles in blastema induction in urodele amphibians. Here, we show that B2FF treatment can induce dermis-derived cells that can participate in multiple cell lineage in limb regeneration. We first established a newt dermis-derived cell line and confirmed that B2FF treatment on the newt cells provided plasticity in cellular differentiation in limb regeneration. To clarify the factors that can provide the plasticity in differentiation, we performed the interspecies comparative analysis..., , # Fluctuation of cellular differentiation in limb regeneration is regulated by Pde4b in urodele amphibians

Dataset DOI: [10.5061/dryad.2rbnzs7p2](10.5061/dryad.2rbnzs7p2)

## Description of the data and file structure

The data were collected from the cultured cells with or without the proteins of interest.  The detail was described in the paper ([https://doi.org/10.1186/s40851-022-00190-6](https://doi.org/10.1186/s40851-022-00190-6)) 

* BF_1_S1_R1_001.fastq.gz ---- Newt cultured cells with BFF 
* BF_2_S2_R1_001.fastq.gz---- Newt cultured cells with BFF (biological replication)
* C1_S3_R1_001.fastq.gz---- Newt cultured cells without BFF (control)
* C2_S4_R1_001.fastq.gz---- Newt cultured cells without BFF (control, biological replication)
* Mouse.BFF_S4_R1_001.fastq.gz---- mouse cultured cells with BFF
* Mouse.BFF.fastq.gz---- mouse cultured cells with BFF (biological replication)
* Mouse.CTRL_S3_R1_001.fastq.gz---- mouse cultured cells without BFF
* Mouse.CTRL.fastq.gz---- mouse cult...,