Gene expression in Su(Hw) null larvae

We examined the effects of loss of su(Hw) on gene expression in both carefully staged 3rd instar larvae (selected at the soft white pre-pupae stage) and wing imaginal discs from these animals. su(Hw) null animals were generated by crossing su(Hw)v mutants with a deficiency (Df(3R)ED5644), homozygous individuals were recognised be the lack of the dominat Tb marker carried on the TM6B marker. Keywords: ChIP-chip RNA from null larvae was compared with a pool of RNA extracted from wild type (Oregon R) larvae at the same stage. To control for genetic background we also examined su(Hw)v and Df(3R)ED5644 heterozygotes with the pool - thus 3 genotypes were examined. For each genotype, 4 independent biological replicates were performed - 2 of these dye swapped with respect to the other two. The same experimental design and gentoypes were used for the analysis in dissected wing imaginal discs.