Characteristics of S. aureus strains with respect to antibiotic resistance, ST and agr types, toxin genotype and production invitro.

MRSA, methicillin-resistant S. aureus; MSSA, methicillin-susceptible S. aureus; ND, not determined; se, staphylococcal enterotoxin;P, proliferative; C, cytotoxic; SSTI, skin and soft tissue infection.#The response profile is determined by the pattern of proliferative responses elicited by different dilutions of bacterial supernatants. A proliferative profile was denoted if all dilutions elicited a proliferative response. A cytotoxic pattern was denoted if a) proliferation was only noted at the highest dilution of the supernatants, and b) the supernatants (1∶50 dilution) resulted in inhibition of PHA-induced responses in co-stimulation experiments. Cells stimulated with supernatants (1∶50 dilution) were also analyzed by flow cytometry and the value in parenthesis shows % cells stained positive for dead cell marker.*Analyses of selected exotoxins including α-toxin (alpha-toxin), SEB (staphylococcal enterotoxin B) and TSST-1 (toxic shock syndrome toxin 1) are assessed in overnight bacterial culture supernatants by luminex. Only positive results are indicated and based on the dilution series response a semi quantitative measure is given as +/−, +, ++, +++.Characteristics of S. aureus strains with respect to antibiotic resistance, ST and agr types, toxin genotype and production invitro.