Repetitive transcranial magnetic stimulation induces cortical layer, brain region, and protocol dependent neural plasticity [Bulk RNA-seq]

Repetitive transcranial magentic stimulation (rTMS) is a non-invasive toll commonly used to study neural plasticity processes and treat neurological disorders. Despite the popularity of rTMS, it is still unclear what neural plasticity mechanisms are induced in the brain regions at and beyond the stimulation site, and how this varies with different rTMS protocols. Here we used spatial transcriptomics to map the neural plasticity mechanisms induced across cortical and sub-cortical regions following intermittent or continuous theta-burst stimulation protocols to the mouse sensorimotor cortex. Our results revealed that rTMS alters the expression of genes related to several cellular processes and neural plasticity mechanisms across the brain which was both brain region and rTMS protocol dependent. In the cortex, the effect of rTMS was not only dependent on the cortical region, but also the cortical layer within each cortical region. These findings uncover the neural plasticity mechanisms induced across the brain following rTMS and help inform how different stimulation protocols can be used to drive specific neural plasticity mechanisms. Whole transcriptome sequencing data generated from RNA extracted from the sensorimotor cortex of wildtype C57BL/6J mice that received a one-off stimulation session of either intermittent theta-burst (iTBS), continuous theta-burst (cTBS) or sham stimulation. A total of five animals were included in each treatment group. Animals were euthanised three hours post-stimulation, after which the brain was removed and the sensorimotor cortex was dissected and extracted for RNA.