Limit of detection between the RT-qPCR (Ct value), RT-LAMP (Tp value) and RT-LAMP LFD assays.

(a) RT-qPCR amplification Ct values corresponding to each of the 10-fold dilutions of RNA standards; (b) RT-LAMP amplification result with Tp values given for each RNA copy number; (c) AGE analysis of RT-LAMP-LFD reaction, spanning the same RNA standards as in (a) and (b); (d) The RT-LAMP-LFD reactions from (c) applied to the LFD device. +/− indicates that out of 4 identical replicates of a given RNA concentration applied to a specific assay (a–d), there were a mixture of positive and negative results.