RNA-sequencing analysis of pro-inflammatory cytokine stimulation of HMC3 microglia and HOG oligodendroglioma cell lines

Here we have stimulated human fetal microglia HMC3 cells and human oligodendroglioma HOG cells with LPS for 24 hours and subsequently with a combination of TNFα and IL-1β for 24 hours , and analyzed their transcriptome-wide response to compare the responsivity of the two cell lines to the pro-inflammatory stimuli. We analyzed the pooled RNA of three wells (technical triplicates) of unstimulated HMC3 cells and pooled RNA of three wells (technical triplicates) of HMC3 cells stimulated with 100 ng/ml LPS for 24 hours and subsequently with 5 ng/ml TNFα and IL-1β for 24 hours. We also analyzed the pooled RNA of three wells (technical triplicates) of unstimulated HOG cells and pooled RNA of three wells (technical triplicates) of HOG cells stimulated with 100 ng/ml LPS for 24 hours and subsequently with 5 ng/ml TNFα and IL-1β for 24 hours.