Raw Data - Atopic Biomarker Changes after Exposure to Porphyromonas gingivalis Lipopolysaccharide: A Small Experimental Study in Wistar Rat

<i>Background</i>. IgE and IgG₄ are implicated in atopic development and clinically utilized as major biomarkers. Atopic responses followingcertain pathogens such as <i>Porphyromonas gingivalis </i>is currently an area of interest for further research. <i>Purpose</i>. The aim of this study is to measure the level of IgE, IgG₄, and IgG₄/IgE ratio periodically after exposure of periodontal pathogen <i>Porphyromonas gingivalis</i>(Pg) lipopolysaccharide (LPS). <i>Methods</i>. We used 16 wistar rats (<i>Rattus norvegicus</i>) randomly subdivided into 4 groups, group 1 were injected by placebo, group 2 by LPS Pg 0.3 µg/mL,group 3 by LPS Pg 1 µg/mL, and group 4 by LPS Pg 3 µg/mL. Sera from both groups were taken from retro-orbital plexus before and after exposure. <i>Results</i>. Level of IgE and IgG₄ increased significantly following exposure of LPS Pg at day-4 and day-11. Greater increase of IgE rather than IgG₄ contributes to rapid decline of IgG₄/IgE ratio, detected in the peripheral blood at day-4 and day-11. <i>Conclusion</i>. Modulation of atopic responses following exposure to LPS Pg is reflected bydecrease in IgG₄/IgE ratio that accompanies an increase of IgE.