Sequencing of methylated cytokines in specific regulatory regions of MafA and Pdx1 genes
收藏DataCite Commons2023-02-22 更新2024-08-18 收录
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https://figshare.com/articles/dataset/Sequencing_of_methylated_cytokines_in_specific_regulatory_regions_of_MafA_and_Pdx1_genes/22137659/1
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The data are sequences of the MafA gene in the 5ʹ flanking region between nucleotides -8118 and -7750 relative to the transcription start site, and of the proximal promoter of the Pdx1 gene, between -275 and +1,to know its cytosines methylation status. Genomic DNA was treated with sodium bisulfite, amplified by PCR and subcloned into pGEM as a vector. The DNA of interest was then extracted from the plasmid and sequenced. The genes analyzed are from DNA obtained from the pancreatic islets of rat pups from mothers fed a control diet and a 50 % protein-restricted diet.
提供机构:
figshare
创建时间:
2023-02-22



