Identification of Burkholderia pseudomallei essential genes using TraDIS

Fastq sequencing files of Transposon-directed insertion site (TraDIS) sequencing of a library of over 500,000 Burkholderia pseudomallei mutants. Genomic DNA were extracted from pools of transposon mutants harvested from different growth conditions. Transposon flanking regions were PCR-amplified using primers set that bind specifically to the 3' end of the transposon. TraDIS libraries were sequenced on Illumina HiSeq2000 platform. Custom sequencing primer was designed to bind to the transposon end and sequence into the adjacent genomic DNA, allowing us to identify the insertion sites down to a single nucleotide. We predicted genes that contain no or very low transposon insertions as "essential".