DNA methylation profiling of TF bound sites using ChIPBS (ChIP Bisulfite Sequencing)

ChIPBS analysis of HepG2 cells was performed using more direct assay approach, to study the methylation status of transcription factor bound sites and elucidate our understanding on TF-DNA methylation crosstalk ChIP followed by Bisulfite sequencing were performed in HepG2 cell lines. Methylation profile was measured for JUND, YY1, ZBTB33, POL2R and TAF1 and compared to the wgbs methylation profile for the same TF bound sites.