NN-T61915

Mass cytometry comprehensive characterization of small samples of human microglia (1000 to 10000 cells). We determined the expression levels of 57 markers on human microglia that were isolated from up to five different brain regions of nine donors. This experiment contains samples stained with antibody panel A and pre-gated to huMG. FCS files were written upon viSNE analysis conducted on 32 markers in Cytobank and contain the tSNE1/2 parameters.
Notes:
Panel A tSNE on CCL2, CD115, CD11b, CD11c, CD124, CD16, CD163, CD192, CD195, CD206, CD33, CD37, CD40, CD45, CD56, CD62L, CD64, CD68, CD86, CX3CR1, Cyclin A, Cyclin B1, EMR1, HLA-DR, IL-10, IRF4, IRF8, Ki-67, P2Y12, TGF-b, TNF-a, TREM-2 CyTOF2 (upgraded to Helios) instrument was tuned according to the manufactures instructions with tuning solution (Fluidigm) and measurement of EQ four element calibration beads (Fluidigm) containing 140/142Ce, 151/153Eu, 165Ho, and 175/176Lu served as a quality control for sensitivity and recovery. EQ four element calibration beads were added at a final concentration of 1:10 of the sample volume to be able to normalize the data to compensate for signal drift and day-to-day changes in instrument sensitivity.