iprscan

Marsupials exhibit highly specialized patterns of reproduction and development, making them uniquely valuable for comparative genomics studies with their sister lineage, eutherian (also known as placental) mammals. However, marsupial genomic resources still lag far behind those of eutherian mammals, limiting our insight into mammalian diversity. Here, we present a series of novel genomic resources for the fat-tailed dunnart (Sminthopsis crassicaudata), a mouse-like marsupial that, due to its ease of husbandry and ex-utero development, is emerging as a laboratory model. To enable wider use, we have generated a multi-tissue de novo transcriptome assembly of dunnart RNA-seq reads spanning 15 tissues. This highly representative transcriptome is comprised of 2,093,982 assembled transcripts, with a mean transcript length of 830 bp. The transcriptome mammalian BUSCO completeness score of 93% is the highest amongst all other published marsupial transcriptomes. Additionally, we report an improved fat-tailed dunnart genome assembly which is 3.23 Gb long, organized into 1,848 scaffolds, with a scaffold N50 of 72.64 Mb. The genome annotation, supported by assembled transcripts and ab initio predictions, revealed 21,622 protein-coding genes. Altogether, these resources will contribute greatly towards characterizing marsupial biology and mammalian genome evolution.

有袋类动物展现出高度特化的繁殖与发育模式，使其在与姊妹谱系哺乳动物（亦称真兽亚纲，或称有胎盘哺乳动物）的比较基因组学研究方面具有独特价值。然而，有袋类动物的基因组资源与有胎盘哺乳动物相比仍存在较大差距，限制了我们对哺乳动物多样性的认识。在此，我们推出一系列针对肥尾袋狸（Sminthopsis crassicaudata）的新型基因组资源，肥尾袋狸是一种类似小鼠的有袋类动物，因其饲养简便和体外发育的特点，正逐渐成为实验室模型。为促进其更广泛的应用，我们已构建了覆盖15种组织的袋狸RNA测序读段的多组织从头转录组组装。这一高度代表性的转录组由2,093,982个组装转录本组成，平均转录本长度为830碱基对。转录组哺乳动物BUSCO完整性得分为93%，在所有已发表的袋狸转录组中是最高的。此外，我们还报告了一个改进的肥尾袋狸基因组组装，其长度为3.23 Gb，组织成1,848个支架，支架N50为72.64 Mb。基因组注释，在组装转录本和从头预测的支持下，揭示了21,622个编码蛋白基因。总之，这些资源将极大地促进对有袋类动物生物学和哺乳动物基因组进化的表征。