RNA sequencing of Hordeum vulgare near isogenic lines. Hordeum vulgare

NILs containing five parental lines, three wild barley genotypes ssp. spontaneum: HID 4 (A), Iraq; HID 64 (B), Turkey; and HID 369 (C), Israel, one ssp. agriocrithon: HID 382(D)) and cv. Morex (ssp. vulgare, USA). Purpose: Variant calling to identifie markers associated with a awn length QTL on the distal part of chromosome 7HL Overall design: Two NILs differing in awn length were sequenced, one having an introgression from HID382 at position 132.01 – 134.21 cM (POPSEQ map) (RD) and the other from Morex (RM).