Effects of CYTOR Knockdown via CRISPRi on K562 cells

The long noncoding RNA CYTOR has been linked to poor prognosis of cancer, this includes chemo- and radioresistance. Knowing the mechanisms of CYTOR can benefit current cancer therapeuitics. To examine the mechanisms of CYTOR on K562, we performed CRISPRi to knock it down. RNA sequencing were then performed on these knocked down and wild type cells. This is followed by differential gene expression analysis to determine genes and lncRNAs that are differentially regulated when CYTOR is silenced. To illustrate how CYTOR affect the transcription of genes and lncRNAs on K562 cells, we profiled 4 replicates of CYTOR knocked down, and wild type K562 cells