Gene expression profile of SmarT-cells compared to control 1G CAR T cells
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE113391
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资源简介：
The objective of this experiment was to compare the transcriptional profile of T cells modified to express CAR, TBBR and 4/7 ICR (i.e. SmarT-cells) against control 1G CAR T cells expressing CD3z endodomain. RNA samples were isolated from trangenic T cells generated from three independent donors. T cells were sorted 5 days post transduction using a SH800S cell sorter (SONY Biotechnology) to normalize for the mean fluorescence intensity (MFI) between transgenic T cells. RNA was isolated at 24 hours after stimulating the transgenic T cells with OKT3, TGFb and IL4 using the RNeasy mini plus kit (QIAGEN) and hybridized to the nCounter PanCancer immune profiling panel (human codeset).
创建时间：
2018-07-26
