Processed expression matrices for GSE344440: sorted NK-92 cells, IgG versus anti-TIGIT (bulk RNA-seq)

Processed expression matrices from the bulk RNA-seq experiment deposited in NCBI GEO under accession GSE344440. Experiment: live NK-92 cells were sorted after 24 h co-culture with Mycobacterium tuberculosis-infected THP-1 macrophages, following pretreatment with IgG (Ctrl1-Ctrl3) or anti-TIGIT (TIGIT-I1 to TIGIT-I3). n = 3 per group; Illumina NovaSeq X Plus, PE150. Files:- 00_sample_group.csv - sample-to-group lookup- 01_count_matrix.csv - raw gene-level counts (20,348 genes x 6 samples)- 02_TPM_matrix.csv - TPM (20,348 genes x 6 samples)- 03_normalised_expression.csv - log2-scale normalised expression, 14,336 expressed genes; input to the differential-expression and WGCNA analyses- 04_WGCNA_datExpr.csv - WGCNA datExpr, 6 samples x 5,000 genes; direct input to WGCNA.R These matrices reproduce Supplementary Figures 4B-4D (GSVA GO heatmap, DEG volcano, GSEA) and 5A-5I (WGCNA) of the manuscript "TIGIT regulates NK-cell adhesion, cytotoxicity and multicellular control of intracellular pathogens in tuberculosis". Raw sequencing reads are in the SRA under the same BioProject as GSE344440. Analysis code is at https://github.com/ywqin829/TB-TIGIT-NK-Manuscript-Code. The sequencing vendor's HTML report and intermediate WGCNA objects are deliberately not included.