FLUID SHEAR STRESS MODULATES ENDOCYTIC PATHWAYS AND JUNCTIONAL TARGETING OF TUMOR-DERIVED EXTRACELLULAR VESICLES IN ENDOTHELIAL CELLS

Here, we investigated TNBC sEV–endothelial interactions using combined in silico and in vitro approaches. Human umbilical vein endothelial cells (HUVECs) were cultured under static or FSS conditions (20 dyn/cm²), followed by proteomic profiling and protein–protein interaction analyses with sEV proteomes. FSS downregulated proliferation- and angiogenesis-associated proteins while upregulating adhesion and cytoskeletal regulators assessed by proteomics. Network analysis identified clathrin- and caveolin-mediated endocytosis (CME and CavME), integrins, and early endosomes as central mediators of sEV uptake.