Performance of β-glucan and qPCR, overall and according to two restrictive approaches of invasive aspergillosis diagnosis with selection of serum samples.

Abbreviations: IA: Invasive Aspergillosis; GM: Aspergillus galactomannan antigenemia; ß-glucan: (1,3)-b-D glucan antigenemia; qPCR: PCR assay targeting Aspergillus fumigatus mitochondrial DNA.
aYouden index is calculated as follows: Sensitivity+Specificity-1; and varies from 0 (no diagnostic accuracy) to 1 (perfect diagnostic accuracy).
bSelection of serum samples after neutropenia/fever onset, and before antifungal therapy, invasive fungal infection, or neutropenia recovery.
cSelection of serum samples collected on the same sample or the consecutive sample of a GM positive result as defined by a GM index ≥0.5 according to the manufacturer’s specifications.