mRNA Profiling of murine 4T1-T breast cancer cells with Asns knocked down after isolation from primary tumors and lung metastases with 6TG. mRNA Profiling of murine 4T1-T breast cancer cells with Asns knocked down after isolation from primary tumors and lung metastases with 6TG

Murine 4T1-T breast cancer cells were infected with retroviral vectors harboring shRNAs for murine or a Renilla, as negative control. Cells were orthotopically injected into NSG mice. After 19 days mice were euthanized and tumors and lungs were removed and digested into single cells. Cells were then placed in culture dishes with 6TG. Surviving cells were then harvested and processed with the NuGen Ovation RNAseq system V2. Libraries were sequenced on an Illumina platform and mapped using Bowtie2 to the mm10 genome prior to transcript quantification using HT-Count. Overall design: 4T1-T breast cancer cells with Asns knocked down after isolation from primary tumors and lung metastases with 6TG