Alan S. Fanning, Brent P. Little, Christopher Rahner, Darkhan Utepbergenov, Zenta Walther, James M. Anderson (2011) CIL:16221, Canis lupus familiaris, epithelial cell. CIL. Dataset

Cell expressing delta U6 ZO-1 constructs show novel freeze fracture strands extending onto the lateral membrane. Stable MDCK cell lines were fixed and analyzed by freeze fracture electron microscopy. Cell lines expressing delta-U6 (C) or ZNG (aa 1-806) (E and F) show bizarre collections of tight junction fibrils that extend from the orthotopic junctional strands or that can be free-floating on the lateral surface (G), which were not present in cells transfected with vector alone (A), ZNU6 (aa 1-888) (D), or in delta U6-transfected cells grown in the presence of DOX (B). Replicas A–F were imaged at 38,000x magnification and replica G at 21,000x magnification. Bar, 500 nm.

表达delta U6 ZO-1结构的细胞展现出新的冷冻断裂条纹，这些条纹延伸至侧膜。稳定的MDCK细胞系经过固定后，通过冷冻断裂电子显微镜进行分析。表达delta-U6（C）或ZNG（aa 1-806）（E和F）的细胞系显示出奇异的紧密连接纤维束集合，这些纤维束从正位连接条纹延伸出来，或者可以自由漂浮在侧表面（G），而在仅转染载体（A）、ZNU6（aa 1-888）（D）或存在于DOX存在下培养的delta U6转染细胞（B）中则不存在。复制品A–F在38,000倍放大下成像，复制品G在21,000倍放大下成像。标尺，500纳米。