James R. LaFountain, Rudolf Oldenbourg (2011) CIL:10724, Nephrotoma, Nephrotoma suturalis, primary spermatocyte. CIL. Dataset

Movie of anaphase B (spindle elongation) of meiosis I in primary spermatocytes of the crane-fly Nephrotoma suturalis.
Time-lapse polarization microscopy using a Nikon
Microphot SA, equipped for liquid crystal polarized light microscopy
(LC-PolScope, CRi, Woburn Massachusetts) 60x/1.4 PlanApo oil immersion objective, 1.4 NA oil imm. condenser, with 2.0x zoom lens. Images captured every 30 sec over 70 min using a QImaging Retigo EXi CCD camera. Raw images were processed
using 4-frame algorithm (Shribak and Oldenbourg, 2003). A movie based on this time series is included in this grouped set.

本数据集记录了蜻蜓目蜉蝣科昆虫 Nephrotoma suturalis 初级精母细胞有丝分裂 I 期中后期纺锤体伸长过程的动态变化。采用尼康 Microphot SA 极化偏振显微镜，配备液晶偏振光显微镜系统（LC-PolScope，CRi，Woburn，马萨诸塞州），搭载 60x/1.4 PlanApo 油浸物镜，1.4 NA 油浸聚光镜以及 2.0x 变焦镜头进行时间序列偏振显微镜观察。利用 QImaging Retigo EXi CCD 相机每隔 30 秒捕捉图像，持续 70 分钟。原始图像经过四帧算法（Shribak 和 Oldenbourg，2003）处理。本组数据集包含基于此时间序列的动态视频。