MinION sequencing for microbiota identification in broilers on-site

Through the application of metagenomics, it is possible to uncover the contribution of microbial communities to gastrointestinal diseases in poultry and can enhance poultry health by circumventing foodborne pathogen colonization of the gut and can also improve food safety measures of the products. Furthermore, the related cost and analysis of WGS techniques make the technique not available to the primary sector currently . For this reason, molecular methods as being developed to detect the bacteria in the field based in DNA sequencing technologies . Consequently, there is a need more practical, cost-effective, long read-data and suitable techniques for routine diagnosis to determine the status of the flocks in a short turnaround time and with the highest sensitivity.