Gut microbiota analysis in WT and Il17d-deficient mice treated with or without colitis

Microbiota dysbiosis has been reported to contribute to the pathogenesis of colitis, to demonstrate whether IL-17D protects against DSS-induced colitis through regulation of microflora, we performed 16S rRNA sequencing in feces from WT and Il17d-deficient mice. Our data indicate that Il17d deficiency results in microbiota dysibiosis in both steady state and DSS-induced colitis. Overall design: Fresh mouse fecal samples were collected and microbial DNA was isolated from stools of WT and Il17d-deficient mice treated with or without colitis by using QiAamp DNA Stool Mini Kit (QIAGEN) according to manufacturer's instructions. DNA concentration was checked with Nanodrop (Thermo Scientific). The V4 regions of the bacteria 16S rRNA gene were amplified by PCR using primers 515F-806R. The 16S rDNA amplicons were sequenced on an Illumina (HiSeq 2500) platform by Beijing Genomics Institute.