Role of TET1-mediated epigenetic modulation in Alzheimer's disease [RNA-Seq]

To investigate the influence the partial loss of Tet1 has on AD pathogenesis in 5xFAD mice, we crossed a Tet1 KO mouse line with the 5xFAD mouse line to generate WT, 5xFAD, Tet1(+/-), and FAD/Tet1(+/-) mice. We then performed gene expression profiling analysis using data obtained from RNA-seq from biological replicates of mice comprised of 4 different genotypes. Comparative gene expression profiling analysis of RNA-seq data for WT, 5xFAD, Tet1(+/-), FAD/Tet1(+/-) mice to determine how the partial loss of Tet1 influences 5xFAD-associated gene expression and pathology.为探究Tet1部分缺失对5xFAD小鼠阿尔茨海默病（AD）发病进程的影响，本研究将Tet1敲除（Tet1 KO）小鼠品系与5xFAD小鼠品系进行杂交，获得野生型（WT）、5xFAD、Tet1(+/-)及FAD/Tet1(+/-)四种基因型的小鼠。随后，我们针对该四类小鼠的生物学重复（biological replicates）样本的RNA测序（RNA-seq）数据开展基因表达谱分析，并通过对上述四种基因型小鼠的RNA-seq数据进行对比基因表达谱分析，以明确Tet1部分缺失对5xFAD相关基因表达与病理进程的影响。