RNA-seq data from tree shrew spermatogonia stem cells with different treatments
收藏科学数据银行2023-10-24 更新2026-04-23 收录
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资源简介：
Total RNA was extracted from undifferentiated tree shrew spermatogonia using TRNzol. Two independent biological samples were prepared. RNA-seq was performed on the Illumina NovaSeq 6000 system. Clean sequence reads were mapped to the tree shrew reference genome (KIZ v3) using TopHat2 software and coding gene expression was calculated using Cufflinks. Cuffdiff software was used to determine differentially expressed genes (DEGs). Gene Ontology (GO) enrichment analysis was performed using online tools (http://geneontology.org/). Heatmaps were draw using the “gplot” package in R.
提供机构：
Kunming Institute of Zoology
创建时间：
2023-10-23
