Direct RNA long read transcriptomic data of 293T cells containing different promoters

We applied direct RNA long read sequencing for characterization of transcripts from constructs inserted into HEK293T mammalian cells with different promoters. Direct RNA sequencing was performed on an Oxford Nanopore GridION device using the Direct Sequencing Kit (SQK-RNA004, date accessed 15 May 2024), MinION RNA flow cell (FLO-MIN00RA), and data pre-processing was performed with MinKNOW (v24.06.10). Direct RNA- long read sequencing on polyclonal 293T cells containing synthetic contructs that differ in promoter identity.