M Pentecost, G Otto, JA Theriot, MR Amieva (2011) CIL:25361, Oryctolagus cuniculus, Listeria monocytogenes. CIL. Dataset

QuickTime movie of a complete optical scan through a rabbit intestinal villus tip infected with Wt-GFP L. monocytogenes (green), and counterstained with phalloidin (red) to visualize the actin cytoskeleton, and with toto-3 (blue) to visualize nuclei. Paraformaldehyde fixed intact tissue blocks were processed without prior embedding and sectioning. The samples were imaged by confocal microscopy where optical sections were taken at 0.5 micron resolution through both the cell monolayers and the intestinal villi. Z-stacks were reconstructed into three dimensions using Volocity software.

完整光学扫描兔肠道绒毛尖端感染有Wt-GFP L. monocytogenes（绿色）的QuickTime电影，并经鬼笔毒素（红色）复染以可视化肌动蛋白细胞骨架，以及用拓托-3（蓝色）以可视化细胞核。未经预先包埋和切片的福尔马林固定完整组织块被处理。样品通过共聚焦显微镜成像，在0.5微米分辨率下对细胞单层和肠道绒毛进行光学切片。使用Volocity软件将Z栈重建为三维结构。