Gene expression in WT, sdg, clf, and sdg clf. Arabidopsis

Polycomb Repressive Complex 2 (PRC2) recognizes Polycomb response elements (PREs) and catalyzes trimethylation of histone H3 on lysine 27 (H3K27me3) for gene silencing. This silencing is counteracted by H3K36 methylation for epigenetic activation of gene expression. The Arabidopsis thaliana H3K36 methyltransferases SET DOMAIN-CONTAINING PROTEIN 7 (SDG7) and SDG8 antagonize PRC2-mediated silencing and establish H3K36 methylation patterns with the general transcription machinery. The sdg7 sdg8 phenotypes are partially rescued by loss of Polycomb function. To understand this relationship, we examined gene expression in WT, sdg, clf, and sdg clf.