GROQ-seq Function Measurements for LacI, RamR, and VanR Transcription Factor Libraries — DAMP

This dataset contains GROQ-seq functional measurements of LacI, RamR, and VanR transcription factor variant libraries (SSVL, epPCR, and SS) assayed against their cognate wild-type operator sequences. This run of the assay was performed at the Design, Automation, Manufacturing, and Processes (DAMP) Laboratory at Boston University using the same protocol as the companion NIST dataset. Side-by-side comparison between the two sites demonstrates strong cross-site reproducibility of the GROQ-seq platform. Part of a broader effort to generate sequence → function data for allosteric transcription factors with applications in synthetic biology, biosensors, and therapeutics.