Molecular perturbations of hepatocytes isolated from nonheart-beating donor rats. Molecular perturbations of hepatocytes isolated from nonheart-beating donor rats

Organs from nonheart-beating donors are attractive for use in cell therapy. Understanding the nature of molecular perturbations following reperfusion/reoxygenation would be highly significant for nonheart-beating donor cells. Nonheart-beating donor rats hepatocytes were studied for global gene expression with Affymetrix microarraays. Overall design: Groups of rats were used for isolating hepatocytes. The experimental conditions included controls (heart-beating donors) and nonheart-beating rats at various intervals after death as test groups. Each group included samples from three different donor animals.