RNA Sequencing Facilitates Quantitative Analysis of differentially expressed genes during human erythroipoiesis

Purpose: The goals of this study are to determine the differentially expreseed non-coding RNAs during human erythropoiesis Methods:gene expression profiling with cells on days 4,8,11,14 during erythroid differentiation, with two replicated using Illumina Genome Analyzer IIx. qRT–PCR validation was performed using TaqMan and SYBR Green assays. Conclusions: ncRNAs were differentially expressed during erythroid differentiation Overall design: mRNA profiles of day4,8,11,14 erythroid cells were generated by deep sequencing, in duplicate.