ClHst1 has a limited and indirect impact on gene expression.

(A) Expression levels were assessed for genes identified by RNA-seq as induced in Clhst1Δ strains. Expression was assessed by quantitative RT-PCR in ClHST1 (LRY2544, white) and four independently constructed Clhst1Δ strains (LRY2623, light gray; LRY2671; medium gray; LRY2672, dark gray; LRY2673, black). Levels of mRNA for each gene were first normalized to ClPRI2 (CLUG_00368) and then expressed relative to the WT ClHST1 strain. (B) Expression of genes repressed in hst1Δ strains was measured as in part A. For fold repression, the inverse of the fold expression was calculated. (C) The association of ClHst1 with promoters of candidate genes was examined by chromatin IP. Anti-myc antibody was used to immunoprecipitate proteins from ClHST1 (LRY2826) or ClHST1-MYC (LRY2858) yeast.