Transcriptomic analysis of severe allergic patch test reactions to different allergens (nickel, 2-hydroxyethylmethacrylate, methylisothiazolinone and formaldehyde)

Background: Immune mechanisms underlying elicitation in allergic contact dermatitis (ACD) are not disclosed. Previous studies have shown a double-faceted nature of ACD with both common biomarkers between different allergens, as well as allergen-specific imprinting, with discordance in terms of relevant involved immune pathways. Objectives: To characterize molecular signatures and their immune mechanisms between different relevant allergens (nickel, 2-hydroxyethylmethacrylate (2-HEMA), methylisothiazolinone (MI), formaldehyde) in strong and extreme patch test reactions of patients without atopic dermatitis using RNA sequencing. Overall design: Samples: 60 (19 controls, 9 "formaldehyde", 10 "2-hydroxyethylmethacrylate", 10 "methylisotiazolinone", 11 "nickel"). RNAseq