File S1 - The Expression of CD90/Thy-1 in Hepatocellular Carcinoma: An In Vivo and In Vitro Study

Combined file. Table S1, List of primers. TS=this study. Table S2, List of antibodies. Figure S1, Expression of CD90, αSMA and VIM in a HCC by IF. Objective magnification: 10X (A) 40X (B). Figure S2, mRNAexpression of CD90, VIM, and ACTA2 in primary liver cancer by RT-qPCR analysis. A. VIM and ACTA2 distribution in normal, liver cirrhosis LC of HCC and HCC. Boxes indicate the percentiles, lines the median au value for each groups (*p+ and CD90- by FACS analysis. - control – positivity. Figure S4, mRNA analysis of epithelial mesenchymal transition (EMT) cells markers. mRNA relative expression was normalized to reference genes 18SRNA and β-actin; the expression of CD90- cells was considered as 1.00 (Students’ t test *p-). Figure S5, Murine mRNA analysis of liver and lung tissues of xenograft in NOD/SCID mice. Afp=alpha fetoprotein, Alb= albumin. Wild type = CTRL mice, XG JHH-6 CD90+ = xenograft injected with JHH-6 CD90+, XG JHH-6 CD90- = xenograft injected with JHH-6 CD90-. mRNA relative expression was normalized to reference genes β-actin. Reference list of the File S1. (PDF)