Deep-Red and Near-Infrared Xanthene Dyes for Rapid Live Cell Imaging

In this work, two xanthene dyes (H-hNR and TF-hNR) have been synthesized by a convenient and efficient method. These two dyes exhibited deep-red and near-infrared emissions, high fluorescence quantum yields, and good photostability. Their structure–optical properties were investigated by X-ray crystal structure analysis and density functional theory calculations. Live cell imaging data revealed that H-hNR and TF-hNR could rapidly stain both A549 and HeLa cells with low concentrations. The excellent photophysical and imaging properties render them as promising candidates for use in live cell imaging.在本项研究中，通过一种便捷高效的方法合成了两种黄色素染料（H-hNR与TF-hNR）。这两种染料表现出深红色及近红外辐射发射、高荧光量子产率及优良的耐光稳定性。其结构-光学性质经X射线晶体结构分析和密度泛函理论计算得到深入研究。活细胞成像数据揭示，H-hNR与TF-hNR在低浓度下能够迅速染色A549和HeLa细胞。卓越的物理光性质及成像特性使它们成为活细胞成像领域的潜在理想候选者。