RELATe enables genome-scale engineering in functional genomics
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https://www.ncbi.nlm.nih.gov/sra/SRP223403
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资源简介：
We developed RELATe (restriction/ligation coupled with Agrobacterium mediated transformation) method to generate sgRNA library from Cryptococcus neoformans genomic DNA, targeting over 98% of the protein-coding genes. High-throughput screen identified 142 potential targets contributing to blood-brain barrier penetration. We validated two targets, SFP1 and WDR1, for our proof of concept demonstration that RELATe is suitable for genome-wide high-throughput screen in fungi and other eukaryotic species for elucidating functional genomics.
创建时间：
2019-09-27
