Transcriptome analysis of nascent hematopoietic cells II

During ontogeny, HSCs or progenitors are generated from endothelial cells through the process known as endothelial-to-hematopoietic transition (EHT). After EHT, hematopoietic cells form cell aggregates, called hematopoietic clusters. To obtain mechanistic insight into HSC specification, we compared the gene expression profiles of hematopoietic clusters between caudal half region and yolk sac. Hlf+ nascent hematopoietic cells (c-Kit+CD45-) were isolated from embryonic day (E)10.5 caudal half and yolk sac, and were subjected to RNA-seq analysis.在个体发生过程中，造血干细胞（Hematopoietic Stem Cells, HSCs）或造血祖细胞通过内皮向造血转化（endothelial-to-hematopoietic transition, EHT）这一过程由内皮细胞分化产生。完成EHT后，造血细胞会形成细胞聚集体，即造血簇。为深入解析造血干细胞特化的分子机制，我们比较了尾半区与卵黄囊中造血簇的基因表达谱。我们从胚胎发育第10.5天（E10.5）的尾半区和卵黄囊中分离出Hlf+新生造血细胞（c-Kit+CD45-），并对其进行RNA测序（RNA-seq）分析。