Bactericidal permeability increasing protein knockout disrupted microbiome of acute colitis mice

Objective: The objective was to study the role of microbiome in the pathogenesis of experimental murine colitis. Methods: We generated bactericidal permeability increasing protein knockout mice, abbreviated as BPI KO mice. Acute colitis was induced in BPI KO mice and wild type abbreviated as WT mice by subjecting the mice to 5% dextran sulfate sodium, abbreviated as DSS. We collected feces from the BPI KO, WT, DSS+BPI KO abbreviated as DSS_KO and DSS+WT abbreviated as DSS_WT mice to assess microbial differences by high-throughput 16S ribosomal DNA gene sequencing. Results: BPI KO mice subjected to DSS showed increased symptoms of experimental colitis. Microbial diversity of the feces from BPI KO mice was lower than that of the WT mice. Microbial diversity of the feces from DSS_KO mice was lower than that of the DSS_WT mice. The heatmap of the genera shows the differences between the various species of flora in the DSS_WT and DSS_KO mice. Conclusions: The BPI KO mice developed worse colitis than WT mice by disrupted microbiome.