Transfection Of Nsd3-Tareting Sirna In H1299 Cells
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<strong>SGC Open Notebook Project to Characterize the HMTase NSD3</strong> <strong>Exp019 Objective: </strong> I have identified a putative phenotype in H1299 cells upon overexpression of the NSD3 short<br> isoform. I am also interested in any phenotype resulting from decreased amounts of NSD3 in cells. To<br> do so, I am first testing conditions for RNAi-mediated knockdown of NSD3 in H1299 cells by treating cells<br> with several concentrations of siRNA and evaluating knockdown by western blotting
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2018-04-16
