Transcription profiling of mouse colon from wild-type and Slc9a3 (NHE3)-deficient animals

Na+/H+ exchanger 3 (NHE3) provides a major route for intestinal Na+ absorption. It has been considered as a target of proinflammatory cytokines and enteropathogenic bacteria and impaired NHE3 expression and/or activity may be responsible for inflammation-associated diarrhea. We used microarray analysis to detail the global program of colonic gene expression in the absence of functional NHE3. Experiment Overall Design: Whole colon was dissected from 6-8 week old NHE3-deficient mice and their wild-type littermates and total RNA isolated for microarray analysis using Affymetrix murine MOE430 2.0 arrays钠氢交换蛋白3（Na+/H+ exchanger 3, NHE3）是肠道钠离子吸收的主要通路。该蛋白曾被认定为促炎细胞因子与肠道致病菌的作用靶点，NHE3的表达或活性受损可能是炎症相关性腹泻的致病机制之一。本研究采用基因芯片分析技术，对功能性NHE3缺失状态下的结肠全局基因表达谱进行了详细解析。实验整体设计：从6-8周龄的NHE3基因敲除小鼠及其野生型同窝仔鼠体内剥离完整结肠组织，提取总RNA，使用Affymetrix小鼠MOE430 2.0基因芯片完成基因芯片分析。